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Cell culture

• Cell culture

• iPS cell culture

• Organoids

Genome editing

• Plasmid cloning

• Transfection

• CRISPR/Cas9

Molecular biology

• Immunostaining

• RT-PCR

• WesternBlotting

Computation

• MATLAB

• Python

• R

Optics

• Three-photon microscope

• Two-photon microscope

Microfabrication

• Photolithography

• Softlithography

• 3D printing

Others

• Equipment

Development

Development tips

Contents

• About
  • Installation of sphinx
    • Sphinx + extensions
• Cell culture
  • Basic cell culture (HEK293T)
    • Cell lines
    • Reagent
    • Protocols
  • iPS cell culture
    • Cell lines
    • Reagent
    • Protocols
  • AAV production with HEK293T
    • Cell lines
    • Materials & Reagents
    • Protocols
  • Lenti virus production with HEK293T
    • Cell lines
    • Reagent
    • Protocols
  • Differentiation of cerebral orgnaoid
    • Reagent
    • Preparation
    • Protocols
• Genome editing
  • Plasmid Cloning
    • Plasmid list
    • Plasmid cloning technique
  • Genome editing
    • Plasmid
    • Plasmid cloning technique
  • Transient transfection
  • Electroporation
  • Virus infection
• Molecular biology
  • DNA isolation
    • Materials & Reagents
    • Protocol
  • RNA isolation
    • Materials & Reagents
    • Protocol
  • RT-PCR, from total RNA
    • Equipments
    • Materials & Reagents
    • Protocol
  • Cryosectioning
    • Materials & Reagents
    • Solution preparation
    • Protocol
  • Flowcytometry & cell sorting
    • Materials & Reagents
    • Protocol
  • Immunostaining
    • Materials & Reagents
    • Protocol
• Computational anlysis
• Optics
  • Multiphoton microscope
    • 2P2
    • 2P3
    • 3P1
• Microfabrication, microfludic device
  • Photolithgraphy
    • Equipments
    • Materials & Reagents
    • Protocols
  • Softlithgraphy
    • Equipments
    • Materials & Reagents
    • Protocols
• Others
  • Sur Lab room and equipment
    • Cell culture room (46-6296, PCLM Wet Lab)
    • Molucular biology room (46-6066)
    • Histlogy room (46-6213)
    • Slice preparation room (46-6215)
    • Two-photon room #1 (46-6221)
    • Two-photon room #2 (46-6222)
    • Two-photon room #3 (46-6226)
    • Confocal microscope room
    • Three-photon microscope room
  • Brian2
  • Helen rotation
  • Rathi training
• Development
  • Matlab
    • VSCode + plugins : alternative to Matlab Editor
    • Links
  • Python
    • VSCode + plugins + pip
    • Anaconda
    • Links
    • Other alternatives
    • Improve the environment
    • Links