# RT-PCR, from total RNA

## Equipments

- Quantstudio 3 (Applied biosystems) 

## Materials & Reagents

- Superscript VILO cDNA syntheses Kit (Thermofisher)
- Total RNA or poly A RNA, mRNA,
- Takara TB Green Premix Ex Taq II (Takara)
- Primer
- 
## Protocol